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IL3 Effector Reporter Cell

I. Background
IL-3（白細(xì)胞介素-3）是一種由活化 T 細(xì)胞、NK 細(xì)胞、肥大細(xì)胞等產(chǎn)生的多效性造血生長因子，核心功能是促進(jìn)多譜系造血祖細(xì)胞存活、增殖與分化，尤其調(diào)控嗜堿性粒細(xì)胞、肥大細(xì)胞、巨噬細(xì)胞等髓系/淋系細(xì)胞發(fā)育，同時(shí)參與炎癥、過敏及腫瘤免疫微環(huán)境重塑。 IL3R（CD123/CD131）基因編碼的受體是白細(xì)胞介素-3（IL-3）的關(guān)鍵受體，由α（CD123）和β（CD131）兩個(gè)亞基組成，屬于細(xì)胞因子受體超家族。α亞基（CD123）特異性結(jié)合 IL- 3，決定受體特異性，而β亞基（CD131，又稱共同β鏈）與 IL-5、GM-CSF 受體共享，形成異二聚體或寡聚體以傳遞信號(hào)。該受體通過激活 JAK-STAT、PI3K-Akt 和 Ras-MAPK 等通路，調(diào)控造血細(xì)胞的增殖、分化及存活，在早期造血調(diào)控中起核心作用，促進(jìn)髓系、紅系等多系祖細(xì)胞的集落形成。

II. Description
IL3 Effector Reporter Cell 報(bào)告基因藥靶模型很好的模擬了體內(nèi) IL3 的信號(hào)轉(zhuǎn)導(dǎo)過程，原理見下圖所示。 Figure 1. IL3 Effector Reporter Cell 細(xì)胞模型原理圖

II. Introduction
Expressed gene:	IL3
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	DMEM+10%FBS+2 μg/ml Puromycin+ 5 μg/ml Blasticidin+ 200 μg/ml Hygromycin B
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

III. Representative Data
Figure 2. Recombinant IL3 Effector Reporter Cell stably expressing IL3. Figure 3. Dose Response of Recombinant Human IL3 in IL3 Effector Reporter Cell(C1). Figure 4. Inhibition of Human IL3 induced Reporter Activity by CD123&CD1D131 Blocking Abs in IL3 Effector Reporter Cell(C1).